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Hello! . At its best, mass-produced CDs cost US$4.25 per gigabyte, with the average Blu-ray or DVD costing over $10 a gigabyte – even for the best quality discs, ensuring that cloning a disc is a pretty expensive procedure.. This is CloneCD, and it’s the ultimate CD ripper, archive-maker, autorip and a transfer program.. Activate the crack, and it’s off to the races. CloneCD uses a “open key”.. is comprised of the ARF protein Gemin4 and must be visualized only when translation is occurring \[[@pone.0178354.ref008], [@pone.0178354.ref011]\]. In contrast, endogenous *bub3Δ* has an almost completely normal fluorescence pattern that is very similar to the wild type, indicating that *bub3Δ* cells are not deficient in [S]{.ul}u[p]{.ul}pressor [K]{.ul}inase (SK) activity. (**B**) Assay for SK activity in wt and *bub3Δ* cells. Cells were grown in 0.2% dextrose medium and treated as indicated. Error bars represent standard error of the mean (SEM) from three independent experiments. p-values (Fisher’s exact test) comparing wild type to *bub3Δ* from three independent experiments are shown.](pone.0178354.g004){#pone.0178354.g004} We next examined *bub3Δ* cells for evidence of defects in G1 arrest and DNA re-replication due to loss of SK activity. Loss of SK activity leads to a cell cycle defect that is manifested by the inability to arrest cells at G1 \[[@pone.0178354.ref006]\]. To determine whether *bub3Δ* cells are defective in G1 arrest, we performed *bub3Δ* assays with nocodazole treatment and compared wild type and *bub3Δ* for defects in cell cycle arrest ([Fig 5A](#pone.0178354.g005){ref-type=”fig”}). Nocodazole was used here because *bub3Δ* cells are sensitive to it. As predicted by loss of SK activity, nocodazole treatment reduced the number
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