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We may be funded by third parties. Sony Vaio PCG-61711w Drivers Download Windows 7: We may be funded by third parties.,,Role of the proapoptotic protein AIF and the anti-apoptotic protein MCL-1 in chemotherapeutic resistance of human breast cancer cells.
Chemotherapy is the standard therapy for patients with advanced breast cancer. However, resistance to chemotherapy remains a major clinical problem. Proapoptotic proteins AIF and BH3-only proteins are considered to be important mediators of apoptosis and may function as drivers of chemotherapeutic resistance. We investigated the role of AIF and BH3-only protein MCL-1 in chemotherapeutic resistance in human breast cancer cells. We measured the expression and localization of AIF, BCL-XL, and MCL-1 proteins in breast cancer cells and analyzed chemotherapeutic sensitivity to etoposide, CPT-11, and doxorubicin. AIF-siRNA and MCL-1-siRNA were used to block expression of the two proteins. AIF and MCL-1 expression levels correlated inversely with IC50 values, and AIF and MCL-1 expression levels were associated with sensitivity or resistance to chemotherapeutic agents. Blocking of AIF expression by AIF-siRNA increased etoposide sensitivity in MCF-7 cells, which express AIF at high levels, but not in MDA-MB-231 cells, which have low expression levels. Similarly, MCL-1 inhibition by siRNA increased etoposide sensitivity in MCF-7 cells but not in MDA-MB-231 cells. AIF is likely to be a potential anti-apoptotic protein for cell survival by modulating chemotherapeutic resistance in breast cancer cells.Q:
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